Ioerror Errno 2 No Such File Or Directory:

The file might be there but may have a different path so try writing the correct and complete path for the file 

Try this

My_file = open(r'Drive:\Dir\Updated.yaml')

Most likely, the problem is that you're using a relative file path to open the file. 

An absolute path is a path that starts with your computer's root directory, for example 'C:\Python\scripts..' if you're on Windows.

A relative path is a python path that does not start with your computer's root directory, and is instead relative to something called the working directory. You can view Python's current working directory by calling os.getcwd().

Use an absolute path to open the file:

file = open(r'C:\path\to\your\file.ext')